06 PCR
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PCR
![](https://photos.thisispiggy.com/file/wikiFiles/8167F823-C4BB-43CE-866B-B42BD50F47A7.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/42DCF9E3-93FF-4A78-B735-88E71F94E042.jpg)
- DNA polymerase requires primer
![](https://photos.thisispiggy.com/file/wikiFiles/E89E53E6-9821-4DB2-9046-7A31ED4DB57F.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/35E25D82-8A26-4AAC-A80A-807912675B89.jpg)
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![](https://photos.thisispiggy.com/file/wikiFiles/F8404E95-BC2F-4C41-838B-44AFCDA6AD27.jpg)
- used for small amount of DNA curculating
- HIV: RNA. Use reverse transcriptase to make copy of virus RNA: cDNA
Blotting
![](https://photos.thisispiggy.com/file/wikiFiles/156D22BD-70C5-41B2-87F4-A5C445FE1A72.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/AFB07AD3-3A70-4B88-8EB9-8930AD6F5F19.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/CD9B6C7C-CD95-4673-8903-6919034D31DE.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/BF1B81F0-62E3-476F-9A80-D5A8D9C0C52F.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/51459822-A37E-4969-9D0D-5221C2ABAAA8.jpg)
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![](https://photos.thisispiggy.com/file/wikiFiles/E99A298B-07B4-4918-9D0A-4B3DC7DF7FED.jpg)
- put a piece of filter paper on top. DNA stick to it
![](https://photos.thisispiggy.com/file/wikiFiles/6D086680-D87A-4D73-B273-F2C55B070AF4.jpg)
- DNA left complementary to probe
![](https://photos.thisispiggy.com/file/wikiFiles/4B590343-F4BA-4245-9496-B4E403FAA69C.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/DDBE3D2C-5171-475A-9862-986D7ECF3C18.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/31CC4631-B75F-463D-B88A-90A1E37B6F3F.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/228F7ADD-C051-4D04-AA5A-24061ADCB349.jpg)
- for characterizing genes
- length of fragments unique to each particular gene
- Restriction nuclease added to Gene A and Gene B: breaking up into different fragments
- Compare unknown gene that break up to gene A or B
![](https://photos.thisispiggy.com/file/wikiFiles/A800EFFE-A8FF-4771-A524-22A7CE5D36BC.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/663FF785-86CF-4A5C-B591-93024ECE0B82.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/D375F772-B492-4296-94E3-AA08E88C16AF.jpg)
- same technique for RNA
- studies gene expression by finding mRNA corresponding to standard
![](https://photos.thisispiggy.com/file/wikiFiles/A1A8B034-08F2-4605-997D-BF4AA7976DB9.jpg)
- use antibody to protein instead of probe
![](https://photos.thisispiggy.com/file/wikiFiles/32440E61-6F91-42DD-A1BA-A8F24C7B0CB8.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/F39612F8-1B48-45FF-9B76-37E711930770.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/E23275CC-7787-4789-AA63-F0F07D6F0A8A.jpg)
Flow Cytometry
![](https://photos.thisispiggy.com/file/wikiFiles/319A475F-1D89-462B-BD9C-F1213E04535F.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/7A9CAFFB-7A18-4115-810A-EB095C90A9B7.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/C422800B-56A8-4B57-B8E6-EFAC565E9975.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/A742D948-785B-4907-8F0D-81293E40F2CA.jpg)
- side and forward scatter
- forward scatter based on cell size
- side scatter based on granularity
![](https://photos.thisispiggy.com/file/wikiFiles/90F5FEFC-045B-41E6-AC9B-34627A91FAB4.jpg)
- lymphocytes: small, little granules
- granulocytes: big, lots of granules
![](https://photos.thisispiggy.com/file/wikiFiles/455B1C8C-0554-43F2-B421-006522A92443.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/395CC7D3-BCEA-4EB8-8D28-F15C0FECCFC2.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/897BEA0D-585C-4DA0-890F-351C286E1345.jpg)
- determine Hb F in maternal circulation = hemorrhage
![](https://photos.thisispiggy.com/file/wikiFiles/48369A35-5A57-4FC1-A521-C297D74B0BC1.jpg)
Elisa
![](https://photos.thisispiggy.com/file/wikiFiles/310174FA-FA22-4145-8519-E3E1B4C9A7F0.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/58C4F442-512E-49EF-A551-2361598D2197.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/8CD26B11-19D7-4C4B-878A-A2044CE241F7.jpg)
- add serum to one of the wells
- plate designed so antigen stick to surface
- wash away serum and left with antigens bound
![](https://photos.thisispiggy.com/file/wikiFiles/08269159-B27C-4335-A563-D3E513EB88F6.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/8D19392E-5263-42F2-A48C-6D79EA4E8BBB.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/FA90468C-08FD-48C4-9BAB-1B023A5A0603.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/96C27F30-690D-44FF-BBB0-226C242B50DA.jpg)
- direct: for every antigen test for, must have an antibody enzyme linked
- indirect: more common
![](https://photos.thisispiggy.com/file/wikiFiles/F70FB462-6459-4BB8-8400-AD65DA10EDC7.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/280FCAAE-A375-436C-8DB7-5972CC52C080.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/464A39E8-A311-48A5-A24F-CAF075C12A30.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/8F857E52-67AA-43D3-B54B-722ABC984DC5.jpg)
- unbound antibody: free antibody from last slide
![](https://photos.thisispiggy.com/file/wikiFiles/37715FC3-5B57-4D8D-89F7-411EB5A69070.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/72297EDF-8ABC-4013-A018-19FE734CBE9D.jpg)
- any HIV antibody in sample will bind to antigen in plate
![](https://photos.thisispiggy.com/file/wikiFiles/096932B4-4B2A-4648-89C0-531CE9755BAF.jpg)
Microassays
![](https://photos.thisispiggy.com/file/wikiFiles/717EDC6B-E09F-4B73-99F3-E863526DE67D.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/7FFAE32A-0938-409F-A934-5A80F499C22E.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/F698AA7B-5FCB-47F5-8DA5-7678B79AF8BF.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/AC91C2FA-4727-44AB-BDB8-0AB373FA9CD2.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/B41AFBD0-2B0E-43FE-8886-D0D936A7DCAE.jpg)
FISH
![](https://photos.thisispiggy.com/file/wikiFiles/4FA2627D-0E1C-482D-9569-B7D534797F9C.jpg)
- like microarray, uses fluorescent
![](https://photos.thisispiggy.com/file/wikiFiles/A573F662-04B8-4F63-8306-A1704C5AEDFC.jpg)
- tell that gene is present on that chromosome
![](https://photos.thisispiggy.com/file/wikiFiles/61208628-E27A-4EB6-A7B5-966086CF1AA1.jpg)
![](https://photos.thisispiggy.com/file/wikiFiles/6B5111EC-AE71-4B0D-AE7F-E4E2BACE8912.jpg)
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