05 Bacterial Genetics
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Growth
- put bacteria in flask, watch growth
- initial no bacteria, then exponential growth, plateau, loss
- no growth. May be growing in size but not dividing
- exponential, log
- antibiotics greatest effect
- dying from not enough nutrients
- death = growth
Gene Transfer
- DNA in environment or lab experiment
- micro labs: genes in culture media for replication
Conjugation
- DNAase degrade DNA freely in culture
- incorporate plasmids in DNA
- can transfer entire genome or partial genome to another bacteria without plasmid
- plasmid transferred and drags portion of bacteria with it
Transduction
Generalized
- DNA potentially incorporated
Specialized
- viral DNA live in bacterial genome for a while
Lytic
- lytic: generalized transduction, lyses bacteria infected
- lysogenic: may later become lytic or just live inside bacteria
Lysogenic
Transposition
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