02 Bacteria Culture

• Blood: enriched (blood) and differential (blood pattern)
• Eosin methylene blue: selective (gm-) and differential (lactose fermenting)

Fastidious bacteria

Blood Agar

• pseudomonas is negative

Chocolate Agar

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• staph aureus beta hemolysis first

VPN

Aka thayer-martin media.

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Bordet-Gengou agar

Aka potato agar.

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• made from potatoes - high in starch

Loeffler's media

• selective for C. dphtheria.

Tellurite plate

• differential for c. diphtheria. Reduces tellurite to tellurium
• Gray black colonies.

Lowenstein-Jensen

Eaton's agar

• culture for mycoplasma pneumonia.

MacConkey's agar

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Eosin-Methylene Blue

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• EMB

Sorbitol MacConkey

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Buffered charcoal yeast extract

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• charcoal for legionella
• classic: nursing home with atypical pneumonia

Sabouraud's agar

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Special growth requirements

Superoxide dismutase and catalase

• O2 radical toxic to bacteria. Must be neutralized by aerobic organisms

Aerobes

Anaerobes

• no O2 in abscess

• bacteroides: appendicitis

• Aspirate vomit
• Clindamycin for anaerobe above diaphragm (lung)
• Metronidazole for anaerobe below diaphragm
• Also can use cipro/quinolone for diverticulitis/appendicitis

Intracellular